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KMID : 0364820170530010049
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Korean Journal of Microbiology
2017 Volume.53 No. 1 p.49 ~ p.54
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Effects of Sus1, a component of TREX-2 complex, on growth and mRNA export in fission yeast
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Bae Soo-Jeong
Yoon Jin-Ho
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Abstract
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Sus1 / ENY2 is a tiny conserved protein that is involved in chromatin remodeling and mRNA biogenesis. Sus1 is associated to two nuclear complexes, the transcriptional coactivator SAGA and the nuclear pore associated TREX2. In fission yeast, Schizosaccharomyces pombe, ortholog of Sus1 / ENY2 was identified from the genome database. Tetrad analysis showed that the S. pombe sus1 is not essential for growth. However, deletion of the sus1 gene caused cold-sensitive growth retardation with slight accumulation of poly(A)+ RNA in the nucleus. And the Sus1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid analysis and co-immunoprecipitation experiment showed that Sus1 interacts with Sac3, another subunit of TREX2 complex. These results suggest that S. pombe Sus1 is also involved in mRNA export from the nucleus as a component of TREX-2 complex.
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KEYWORD
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fission yeast S. pombe, mRNA export, sus1, TREX-2 complex
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